Gene name |
SPAC1783.06c |
Gene ID |
05/B01 |
Gene synonyms/obsolete |
|
Gene product |
involved in autophagy;
involved in cytoplasm to vacuole targeting |
Entry clone |
Cloned |
ORF length (unspliced) |
517 |
ORF length (spliced) |
399 |
Entry clone length |
517 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
393A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1783.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACGGGATTAGTCGATCA |
Rev primer name |
SPAC1783.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACCGAAAGCAACGTTGATA |
Amino acid length |
132 |
Molecular weight |
15.1 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGLPMNSSLVL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |