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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1142.05
Gene ID 05/B11
Gene synonyms/obsolete
Gene product copper ion transporter; similar to Sp ctr4 (paralog)
Entry clone Cloned
ORF length (unspliced) 522
ORF length (spliced)
Entry clone length 522
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1142.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCTTAAGTAAGATGAG
Rev primer name SPAC1142.05.Rv
Rev primer SEQ AGAAAGCTGGGTATCTTGATTCGCAGCATCCA
Amino acid length 173
Molecular weight 19
Isoelectric point (calc.) 9.2
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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