Gene name |
SPAC4F8.10c |
Gene ID |
05/C02 |
Gene synonyms/obsolete |
|
Gene product |
calponin family;
involved in actin cytoskeletal organization |
Entry clone |
Cloned |
ORF length (unspliced) |
525 |
ORF length (spliced) |
|
Entry clone length |
525 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4F8.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACGTCTCAACTTGAAAA |
Rev primer name |
SPAC4F8.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATACATATTTCCGGTCGGG |
Amino acid length |
174 |
Molecular weight |
20.1 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHTKLNAQLDL |
Localization (YFP) |
nucleus>cytosol;
periphery at cell tip and site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |