Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3A12.10
Gene ID 05/D03
Gene synonyms/obsolete rpl2001; yl17b; rpl18a-2; rpl20-1; rpl20
Gene product 60S ribosomal protein L20a
Entry clone Cloned
ORF length (unspliced) 531
ORF length (spliced)
Entry clone length 531
No. of intron 0
Sequence status Finished
Sequence results 355A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3A12.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCACTGAAGGAGTATCA
Rev primer name SPAC3A12.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGTAGAAAGTGCTGGGGCGA
Amino acid length 176
Molecular weight 20.5
Isoelectric point (calc.) 11
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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