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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31G5.07
Gene ID 05/D07
Gene synonyms/obsolete
Gene product involved in conjugation
Entry clone Cloned
ORF length (unspliced) 537
ORF length (spliced)
Entry clone length 537
No. of intron 0
Sequence status Finished
Sequence results 458G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31G5.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTGTGTACTTTCTGCTAA
Rev primer name SPAC31G5.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATACTTTGTTGTTGAGGAT
Amino acid length 178
Molecular weight 19.3
Isoelectric point (calc.) 10.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 4
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LMGVVSSLPL
Localization (YFP) ER
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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