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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC16C4.16c
Gene ID 05/D11
Gene synonyms/obsolete
Gene product hypothetical protein; possibly fungal specific; similar to N. crassa 65E11.120; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 538
ORF length (spliced) 486
Entry clone length 538
No. of intron 1
Sequence status Finished
Sequence results 471A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC16C4.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATATCGTTGCAGAGAC
Rev primer name SPCC16C4.16.Rv
Rev primer SEQ AGAAAGCTGGGTACAAGTTATACCTTTTAGAT
Amino acid length 161
Molecular weight 18.4
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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