| Gene name |
SPAC13D6.02c |
| Gene ID |
05/E01 |
| Gene synonyms/obsolete |
byr3 |
| Gene product |
zinc finger protein;
zf-CCHC type (zinc knuckle); involved in mating pheromone
recognition pathway; deletion mutant results in conjugation
defects; involved in conjugation; cellular nucleic acid
binding protein homolog; overexpression suppresses sporulation
defects of ras1 mutant |
| Entry clone |
Cloned |
| ORF length (unspliced) |
540 |
| ORF length (spliced) |
|
| Entry clone length |
540 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC13D6.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGTCTGAATCTGTTCC |
| Rev primer name |
SPAC13D6.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCCTCAATTACAGGAGAG |
| Amino acid length |
179 |
| Molecular weight |
19.3 |
| Isoelectric point (calc.) |
7.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |