| Gene name |
SPAC343.03 |
| Gene ID |
05/E08 |
| Gene synonyms/obsolete |
|
| Gene product |
anaphase-promoting
complex (APC); involved in cyclin degradation (required);
involved in metaphase-anaphase transition (required); zinc
finger protein; zf-C3HC4 type (RING finger); ubiquitin ligase
(E3); conserved eukaryotic protein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
545 |
| ORF length (spliced) |
285 |
| Entry clone length |
545 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
223T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC343.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGGTGAAAATACTGAG |
| Rev primer name |
SPAC343.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTGTGTTTCAGACTTTTCA |
| Amino acid length |
94 |
| Molecular weight |
10.5 |
| Isoelectric point (calc.) |
7.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |