Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F8.05
Gene ID 05/F01
Gene synonyms/obsolete isp3; meu4
Gene product involved in sexual differentiation; meiotic expression upregulated; no apparent orthologs
Entry clone Cloned
ORF length (unspliced) 549
ORF length (spliced)
Entry clone length 549
No. of intron 0
Sequence status Finished
Sequence results 465A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F8.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGTCTCGGAAATTTGTG
Rev primer name SPAC1F8.05.Rv
Rev primer SEQ AGAAAGCTGGGTATGCGGCAGGAGCAGCAGGA
Amino acid length 182
Molecular weight 19.6
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Leica

Image information
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