| Gene name |
SPBC36.10 |
| Gene ID |
05/G01 |
| Gene synonyms/obsolete |
|
| Gene product |
phenylalanyl-tRNA
synthetase (alpha subunit); phenylalanine-tRNA ligase
activity; involved in protein biosynthesis |
| Entry clone |
Cloned |
| ORF length (unspliced) |
555 |
| ORF length (spliced) |
|
| Entry clone length |
555 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC36.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGATTTTTGAAAGTTG |
| Rev primer name |
SPBC36.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCGGTCGCTTCAGAAGAA |
| Amino acid length |
184 |
| Molecular weight |
21 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol; cytoplasmic
dots; mitochondrion? |
| Comments for localization |
a lot of moving dots;
aggregates by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |