| Gene name |
SPBC24C6.11 |
| Gene ID |
05/G03 |
| Gene synonyms/obsolete |
cwf14 |
| Gene product |
G10 protein; complexed
with Cdc5p; involved in mRNA splicing (implicated); involved
in cell cycle regulation (implicated) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
555 |
| ORF length (spliced) |
441 |
| Entry clone length |
555 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
472T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC24C6.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTCGTCTTCGAACGTC |
| Rev primer name |
SPBC24C6.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCACAGCTTGCACAACCA |
| Amino acid length |
146 |
| Molecular weight |
17 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Leica |