Gene name |
SPAC17H9.07 |
Gene ID |
05/G08 |
Gene synonyms/obsolete |
|
Gene product |
protein signal
sequence binding activity; involved in intracellular protein
transport; involved in protein-ER targeting; involved in
SRP-dependent cotranslational membrane targeting |
Entry clone |
Cloned# |
ORF length (unspliced) |
557 |
ORF length (spliced) |
363 |
Entry clone length |
557 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC17H9.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTTACCTGCAAACTGT |
Rev primer name |
SPAC17H9.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTCTTCTTCCCCCTTTTT |
Amino acid length |
120 |
Molecular weight |
13.4 |
Isoelectric point (calc.) |
10.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
111 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>nucleus=cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle
(nucleolus>nucleus>cytosol) |
Microscope used for
observation |
DeltaVision |