| Gene name |
SPBP23A10.16 |
| Gene ID |
05/G11 |
| Gene synonyms/obsolete |
sdh4 |
| Gene product |
succinate
dehydrogenase (ubiquinone) (cytochrome b small subunit);
membrane anchor subunit; inner membrane translocase component;
involved in mitochondrial electron transport, succinate to
ubiquinone; involved in tricarboxylic acid cycle; involved in
protein-membrane targeting; involved in protein-mitochondrial
targeting; protein binding activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
561 |
| ORF length (spliced) |
|
| Entry clone length |
561 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBP23A10.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCAATAATGTGGACAC |
| Rev primer name |
SPBP23A10.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGATTTCCAAAGCTTTTTA |
| Amino acid length |
186 |
| Molecular weight |
20.7 |
| Isoelectric point (calc.) |
10.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
141 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |