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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC338.04
Gene ID 05/H07
Gene synonyms/obsolete cid2
Gene product caffeine induced death protein; involved in DNA replication checkpoint
Entry clone Cloned
ORF length (unspliced) 563
ORF length (spliced) 504
Entry clone length 563
No. of intron 1
Sequence status Finished
Sequence results 145T:C / 528A:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC338.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATGAAGAAAAACGGGG
Rev primer name SPCC338.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGTCTTTATAAGTGTCTCTC
Amino acid length 167
Molecular weight 19.6
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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