| Gene name |
SPAC27E2.04c |
| Gene ID |
05/H08 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan |
| Entry clone |
Cloned |
| ORF length (unspliced) |
564 |
| ORF length (spliced) |
|
| Entry clone length |
564 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
464T:C / 495G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC27E2.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGGCCGACATCAGGATG |
| Rev primer name |
SPAC27E2.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAAACAATATGTAAAGATG |
| Amino acid length |
187 |
| Molecular weight |
22.4 |
| Isoelectric point (calc.) |
10.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKEIYCRLKI/LLPPFSHLLVL/LLEFITLFI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |