| Gene name |
SPAC1782.04 |
| Gene ID |
06/A06 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan |
| Entry clone |
Cloned
(Re-cloned) |
| ORF length (unspliced) |
570 |
| ORF length (spliced) |
528 |
| Entry clone length |
570 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC1782.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTACGAAGAGGTGCGCT |
| Rev primer name |
SPAC1782.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTTTCCTAGACGTTTCCTA |
| Amino acid length |
175 |
| Molecular weight |
19.8 |
| Isoelectric point (calc.) |
10.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
146/147/160 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKGLNGLSL/LISITPLDL |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|