Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPCC613.03
Gene ID 06/A08
Gene synonyms/obsolete
Gene product EF hand motif; involved in secretory pathway
Entry clone Cloned#
ORF length (unspliced) 570
ORF length (spliced)
Entry clone length 570
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC613.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGTTTTCTACCGTAGG
Rev primer name SPCC613.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAATGCTCACACGACGATAC
Amino acid length 189
Molecular weight 22.4
Isoelectric point (calc.) 5.6
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER; Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.