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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G8.09
Gene ID 06/A10
Gene synonyms/obsolete
Gene product SET1 complex; involved in transcriptional silencing; involved in regulation of chromatin remodelling; non-essential
Entry clone Cloned
ORF length (unspliced) 571
ORF length (spliced) 387
Entry clone length 571
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17G8.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATAGGGATGTAACACT
Rev primer name SPAC17G8.09.Rv
Rev primer SEQ AGAAAGCTGGGTATTTGCGCCCATCCATGGTT
Amino acid length 128
Molecular weight 14.9
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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