| Gene name |
SPAC13C5.06c |
| Gene ID |
06/B12 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
sequence orphan |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
578 |
| ORF length (spliced) |
534 |
| Entry clone length |
578 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC13C5.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGGGTTTTGTTGTCAT |
| Rev primer name |
SPAC13C5.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACGTAAGGAATATGGTTG |
| Amino acid length |
177 |
| Molecular weight |
20 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER; cytoplasmic dots;
Golgi? |
| Comments for localization |
ER-Golgi? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |