| Gene name |
SPAC3A12.14 |
| Gene ID |
06/C03 |
| Gene synonyms/obsolete |
cam1 |
| Gene product |
calmodulin; EF hand
motifs; calcium binding protein; essential; involved in
chromosome segregation (required) |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
579 |
| ORF length (spliced) |
453 |
| Entry clone length |
579 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
Spliced just
downstream of ATG. |
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC3A12.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTATGTTTATTATATTG |
| Rev primer name |
SPAC3A12.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTGGAAGAAATGACACGA |
| Amino acid length |
150 |
| Molecular weight |
16.9 |
| Isoelectric point (calc.) |
3.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |