Gene name |
SPBC146.04 |
Gene ID |
06/C05 |
Gene synonyms/obsolete |
|
Gene product |
Erv1/Alr family;
involved in mitochondrial biogenesis; similar to Sp
SPAC3G6.08 |
Entry clone |
Cloned |
ORF length (unspliced) |
579 |
ORF length (spliced) |
|
Entry clone length |
579 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
151A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC146.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATCCTTAATAGAAGAAT |
Rev primer name |
SPBC146.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACCAGAATAGTCGTGATCT |
Amino acid length |
192 |
Molecular weight |
22.3 |
Isoelectric point (calc.) |
7.1 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots;
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|