Gene name |
SPCC2H8.05c |
Gene ID |
06/D01 |
Gene synonyms/obsolete |
SPCC63.01c |
Gene product |
hypothetical coiled
coil protein; hypothetical protein; sequence orphan; predicted
coiled-coil region |
Entry clone |
Cloned |
ORF length (unspliced) |
585 |
ORF length (spliced) |
|
Entry clone length |
585 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
24G:A / 307T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC2H8.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCCTGATGATGTCTC |
Rev primer name |
SPCC2H8.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTGCTTATATGATCTGAT |
Amino acid length |
217 |
Molecular weight |
24.6 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB; nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |