Gene name |
SPAC1782.02c |
Gene ID |
06/D03 |
Gene synonyms/obsolete |
|
Gene product |
involved in
mitochondrial DNA maintenance |
Entry clone |
Cloned |
ORF length (unspliced) |
586 |
ORF length (spliced) |
486 |
Entry clone length |
586 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1782.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGTTCTCTTTTCGAGC |
Rev primer name |
SPAC1782.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGATTCTTCAACTTCACGT |
Amino acid length |
161 |
Molecular weight |
18.3 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSGLVFLIL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |