| Gene name |
SPAC9G1.01 |
| Gene ID |
06/D06 |
| Gene synonyms/obsolete |
SPAC3H1.14 |
| Gene product |
hypothetical protein;
involved in vacuolar import and degradation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
588 |
| ORF length (spliced) |
|
| Entry clone length |
588 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
27T:C / 224A:G /
435A:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC9G1.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAGGATGTTCATTTCT |
| Rev primer name |
SPAC9G1.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTGAAATTCAATGACCGGA |
| Amino acid length |
195 |
| Molecular weight |
22.6 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|