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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC18G6.14c
Gene ID 06/D07
Gene synonyms/obsolete rps7
Gene product 40S ribosomal protein S7
Entry clone Cloned
ORF length (unspliced) 588
ORF length (spliced)
Entry clone length 588
No. of intron 0
Sequence status Finished
Sequence results 169A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC18G6.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGCTCTCAACAAGAT
Rev primer name SPAC18G6.14.Rv
Rev primer SEQ AGAAAGCTGGGTACAAGCCCTCGCCGGTAGCA
Amino acid length 195
Molecular weight 21.9
Isoelectric point (calc.) 10.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 97
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus>cytosol
Comments for localization
Effect of LMB on protein localization changed to: nucleus>>cytosol
Microscope used for observation Leica

Image information
YFP 2 images) See all images
LMB 2 images) See all images

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