| Gene name |
SPAC2E1P3.05c |
| Gene ID |
06/E05 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein;
fungal cellulose binding domain; GPI anchored protein;
glycoprotein |
| Entry clone |
Cloned |
| ORF length (unspliced) |
594 |
| ORF length (spliced) |
|
| Entry clone length |
594 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
81A:T / 274G:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC2E1P3.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTTACTCAAAGTTTGTT |
| Rev primer name |
SPAC2E1P3.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATAGCCGGTAACATTCCAG |
| Amino acid length |
197 |
| Molecular weight |
20.1 |
| Isoelectric point (calc.) |
4 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFLTVLTLAL |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
Confocal
|