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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3D6.08c
Gene ID 06/E10
Gene synonyms/obsolete
Gene product small nuclear ribonucleoprotein (Sm protein); RNA-binding protein; involved in mRNA decay; involved in rRNA processing; involved in deadenylation-dependent decapping
Entry clone Cloned
ORF length (unspliced) 596
ORF length (spliced) 423
Entry clone length 596
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3D6.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATCAAGCTACACAGAT
Rev primer name SPBC3D6.08.Rv
Rev primer SEQ AGAAAGCTGGGTAATACAAATCATCATGTCCG
Amino acid length 140
Molecular weight 16.1
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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