Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1296.04
Gene ID 06/G02
Gene synonyms/obsolete
Gene product very hypothetical protein; dysferlin-C terminal domain
Entry clone Cloned
ORF length (unspliced) 1142
ORF length (spliced) 747
Entry clone length 1142
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments ORF prediction was changed.
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1296.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACGGAATCTGAAAGGAC
Rev primer name SPAC1296.04.Rv
Rev primer SEQ AGAAAGCTGGGTAATCCTGGGGAGTTGGAGAT
Amino acid length 248
Molecular weight 28.5
Isoelectric point (calc.) 6.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 38/32
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery
Comments for localization a few cytoplasmic dots
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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