Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1952.12c
Gene ID 07/A02
Gene synonyms/obsolete csn71; csn7a; csn7
Gene product COP9/signalosome complex; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 618
ORF length (spliced)
Entry clone length 618
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1952.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGAAAAGATTTCACA
Rev primer name SPAC1952.12.Rv
Rev primer SEQ AGAAAGCTGGGTATAGATCAAAGTCAGTATCG
Amino acid length 205
Molecular weight 23.8
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 193
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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