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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP35G2.12
Gene ID 07/A03
Gene synonyms/obsolete
Gene product nucleoside diphosphate-sugar hydrolase; Nudix family hydrolase
Entry clone Cloned
ORF length (unspliced) 618
ORF length (spliced)
Entry clone length 618
No. of intron 0
Sequence status Finished
Sequence results 443A:G / 571A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP35G2.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCCAAGATCAAAAACC
Rev primer name SPBP35G2.12.Rv
Rev primer SEQ AGAAAGCTGGGTAAGACGACAAGTATTTAAGA
Amino acid length 205
Molecular weight 23
Isoelectric point (calc.) 4.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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