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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP35G2.02
Gene ID 07/A10
Gene synonyms/obsolete
Gene product conserved hypothetical protein; eukaryotic conserved protein; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 624
ORF length (spliced)
Entry clone length 624
No. of intron 0
Sequence status Finished
Sequence results 384A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP35G2.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGGCCCTCACCATTG
Rev primer name SPBP35G2.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATAGTAAGAACGGAAAACC
Amino acid length 207
Molecular weight 23.3
Isoelectric point (calc.) 4.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYQNLQNLNI
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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