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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23H4.11c
Gene ID 07/C12
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 638
ORF length (spliced) 567
Entry clone length 638
No. of intron 1
Sequence status Finished
Sequence results 154A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23H4.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATATGGAGGAACAAAT
Rev primer name SPAC23H4.11.Rv
Rev primer SEQ AGAAAGCTGGGTAGTCTAATTCTTCTATTGCA
Amino acid length 188
Molecular weight 21.9
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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