Gene name |
SPAC1952.08c |
Gene ID |
07/D08 |
Gene synonyms/obsolete |
|
Gene product |
conserved hypothetical
protein; possibly involved in RNA processing from Sc 2-hybrid
data |
Entry clone |
Cloned |
ORF length (unspliced) |
648 |
ORF length (spliced) |
573 |
Entry clone length |
648 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
(-5)C:T / 6C:T |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1952.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCTCAAAAGTCGATTC |
Rev primer name |
SPAC1952.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAATGGAGTCTCATCTCCT |
Amino acid length |
190 |
Molecular weight |
21.6 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
Microscope used for
observation |
DeltaVision |