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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP8B7.28c
Gene ID 07/D12
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 648
ORF length (spliced)
Entry clone length 648
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP8B7.28.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTTAAGAGTCGAAA
Rev primer name SPBP8B7.28.Rv
Rev primer SEQ AGAAAGCTGGGTATACAATTTGTTTTTCTTTT
Amino acid length 215
Molecular weight 25
Isoelectric point (calc.) 5.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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