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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC4G3.13c
Gene ID 07/E01
Gene synonyms/obsolete
Gene product CUE domain protein; involved in the ubiquitin system; possibly recruits a soluble ubiquitin-conjugating enzyme to the membrane
Entry clone Cloned
ORF length (unspliced) 648
ORF length (spliced)
Entry clone length 648
No. of intron 0
Sequence status Finished
Sequence results 612A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC4G3.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAACCAGAGCAATTAGC
Rev primer name SPCC4G3.13.Rv
Rev primer SEQ AGAAAGCTGGGTAATTCTTGTCTTGTTTTTCT
Amino acid length 215
Molecular weight 23.5
Isoelectric point (calc.) 9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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