Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17A5.17
Gene ID 07/E07
Gene synonyms/obsolete SPAC1610.01
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 654
ORF length (spliced)
Entry clone length 654
No. of intron 0
Sequence status Finished
Sequence results 18C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17A5.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTACCAAAATTGACTC
Rev primer name SPAC17A5.17.Rv
Rev primer SEQ AGAAAGCTGGGTACTCTGTGCGATGCCATTTC
Amino acid length 217
Molecular weight 24.9
Isoelectric point (calc.) 4.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.