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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17G8.07
Gene ID 07/E08
Gene synonyms/obsolete
Gene product involved in transcriptional regulation; chromatin associated; human homologue GAS41 is glioma amplified; predicted C-terminal coiled-coil region; histone-like transcription factor family
Entry clone Cloned
ORF length (unspliced) 654
ORF length (spliced)
Entry clone length 654
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17G8.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCACCTAATACACGAGT
Rev primer name SPAC17G8.07.Rv
Rev primer SEQ AGAAAGCTGGGTATTCGACAGGTGTGGAGCTT
Amino acid length 217
Molecular weight 24.9
Isoelectric point (calc.) 5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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