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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC4B3.08
Gene ID 07/F01
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 657
ORF length (spliced)
Entry clone length 657
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC4B3.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATCCATTTGAGGGCCG
Rev primer name SPCC4B3.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGGAAGTTGCATGAAGACAT
Amino acid length 218
Molecular weight 24.6
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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