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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP8B7.12c
Gene ID 07/F07
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 663
ORF length (spliced)
Entry clone length 663
No. of intron 0
Sequence status Finished
Sequence results 593T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP8B7.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATAATAACGACCAAAT
Rev primer name SPBP8B7.12.Rv
Rev primer SEQ AGAAAGCTGGGTAATAGGAACTATAATTGTCT
Amino acid length 220
Molecular weight 24.9
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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