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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17A5.10
Gene ID 07/G08
Gene synonyms/obsolete
Gene product conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 675
ORF length (spliced)
Entry clone length 675
No. of intron 0
Sequence status Finished
Sequence results 556G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17A5.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGATGATTCTGTCCC
Rev primer name SPAC17A5.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGTAGGGAATTTTTCCAACA
Amino acid length 224
Molecular weight 24
Isoelectric point (calc.) 9.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots, especially at site of septum formation; cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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