Gene name |
SPAC23A1.15c |
Gene ID |
07/H05 |
Gene synonyms/obsolete |
|
Gene product |
eukaryotic conserved
protein; membrane glycoprotein; involved in intracellular
protein transport and endocytosis |
Entry clone |
Cloned# |
ORF length (unspliced) |
681 |
ORF length (spliced) |
|
Entry clone length |
681 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC23A1.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCGACGTTCTGAATGC |
Rev primer name |
SPAC23A1.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCGAAGAAACCAAAGAAAC |
Amino acid length |
226 |
Molecular weight |
26.2 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi; cytoplasmic
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |