| Gene name |
SPCC1235.08c |
| Gene ID |
07/H07 |
| Gene synonyms/obsolete |
pdh1 |
| Gene product |
conserved eukaryotic
protein; similar to S. cerevisiae YOL107W; predicted
N-terminal signal sequence; 7 predicted transmembrane helices;
essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
681 |
| ORF length (spliced) |
|
| Entry clone length |
681 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1235.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATCATTTCTCCAAGTT |
| Rev primer name |
SPCC1235.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATAGGAAGCTGAGTATGA |
| Amino acid length |
226 |
| Molecular weight |
26 |
| Isoelectric point (calc.) |
7.8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
7 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSKYLIYLFL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |