Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC869.08
Gene ID 08/A11
Gene synonyms/obsolete
Gene product protein-L-isoaspartate O-methyltransferase; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 693
ORF length (spliced)
Entry clone length 693
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC869.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTCTGGAGTTTTAACCT
Rev primer name SPAC869.08.Rv
Rev primer SEQ AGAAAGCTGGGTAGTAATCTGATGAGTCATCT
Amino acid length 230
Molecular weight 25.3
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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