| Gene name |
SPCC737.01c |
| Gene ID |
08/B02 |
| Gene synonyms/obsolete |
SPCC297.06c |
| Gene product |
hypothetical protein;
sequence orphan; predicted coiled-coil |
| Entry clone |
Cloned |
| ORF length (unspliced) |
693 |
| ORF length (spliced) |
|
| Entry clone length |
693 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
344A:G / 381A:C /
510T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC737.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGGGGAAAATCAATTA |
| Rev primer name |
SPCC737.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTAGAAAAACCTTGCAAC |
| Amino acid length |
230 |
| Molecular weight |
27.6 |
| Isoelectric point (calc.) |
10.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
bright and large
nuclear dots by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |