| Gene name |
SPCC1682.04 |
| Gene ID |
08/B10 |
| Gene synonyms/obsolete |
|
| Gene product |
EF hand motifs;
involved in spindle pole body duplication (IMP); calcium
binding protein; Caltractin-cdc31 subfamily; involved in Sad1p
localization (required); essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
697 |
| ORF length (spliced) |
531 |
| Entry clone length |
697 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
140A:G / 632G:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1682.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTGCTAACGCACGGGC |
| Rev primer name |
SPCC1682.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCTTCATCCATCATAATT |
| Amino acid length |
176 |
| Molecular weight |
20.4 |
| Isoelectric point (calc.) |
4.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB;
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |