Gene name |
SPAC57A10.09c |
Gene ID |
08/B11 |
Gene synonyms/obsolete |
|
Gene product |
nonhistone chromosomal
protein; HMG box; involved in establishment and/or maintenance
of chromatin architecture; involved in transcriptional
regulation from PolII promoter |
Entry clone |
Cloned |
ORF length (unspliced) |
699 |
ORF length (spliced) |
327 |
Entry clone length |
699 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC57A10.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTAGAGCCGCAAAATC |
Rev primer name |
SPAC57A10.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCCTCTTTGGCGGCAGCA |
Amino acid length |
108 |
Molecular weight |
12.3 |
Isoelectric point (calc.) |
10.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|