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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F7.10
Gene ID 08/E04
Gene synonyms/obsolete
Gene product hydantoin racemase; similar to Sp SPCC576.02 (paralog)
Entry clone Cloned#
ORF length (unspliced) 717
ORF length (spliced)
Entry clone length 717
No. of intron 0
Sequence status Finished
Sequence results 328G:A
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1F7.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTCGCATCCTTGTCAT
Rev primer name SPAC1F7.10.Rv
Rev primer SEQ AGAAAGCTGGGTAGTCGGAACCTACAGCTTGA
Amino acid length 238
Molecular weight 25.4
Isoelectric point (calc.) 5.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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