Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCP1E11.10
Gene ID 08/E08
Gene synonyms/obsolete
Gene product ankyrin-repeat protein
Entry clone Cloned
ORF length (unspliced) 720
ORF length (spliced) 624
Entry clone length 720
No. of intron 2
Sequence status Finished
Sequence results 339A:C / 533T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCP1E11.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTACAACAACTCCAAA
Rev primer name SPCP1E11.10.Rv
Rev primer SEQ AGAAAGCTGGGTAATTTGAGCGTGGACGAACG
Amino acid length 207
Molecular weight 22.8
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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