| Gene name |
SPBC19C2.15c |
| Gene ID |
08/G01 |
| Gene synonyms/obsolete |
SPBC2F12.16 |
| Gene product |
eukaryotic conserved
protein; PTPLA; protein tyrosine phosphatase-like protein; Sc
YJL097W is null-lethal |
| Entry clone |
Cloned |
| ORF length (unspliced) |
729 |
| ORF length (spliced) |
627 |
| Entry clone length |
729 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC19C2.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAAAATTTGTAAGAC |
| Rev primer name |
SPBC19C2.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGGTTCTTCTGGCAGCAGCC |
| Amino acid length |
208 |
| Molecular weight |
24 |
| Isoelectric point (calc.) |
10.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
4 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVRWVQTLAI |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |