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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC2C4.08
Gene ID 08/G08
Gene synonyms/obsolete
Gene product hypothetical protein
Entry clone Cloned
ORF length (unspliced) 732
ORF length (spliced)
Entry clone length 732
No. of intron 0
Sequence status Finished
Sequence results 133G:A / 438A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC2C4.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACAGATTGCAAGCAAA
Rev primer name SPAC2C4.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAATTAGACAACGTGAAT
Amino acid length 243
Molecular weight 28
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAKMLTHLNI
Localization (YFP) nucleolus>>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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